The lattice light-sheet microscope is a refinement on the idea of selective-plane illumination microscopy (SPIM), where sample excitation is a thin sheet of light, sectioning the sample and reducing background fluorescence, photo-bleaching, and photo-toxicity.  Sampling frame rates are dramatically improved by widefield imaging of the illuminated plane with the detection objective oriented normal to the excitation plane.This instrument will enable acquisition of spatiotemporal information in living cells not possible on any existing microscope systems and will play an important role in accessing information about molecular organization and dynamics.

Location: SDSMT
Contact: Robert Anderson

Lattice Light Sheet

(A) Lattice light-sheet microscope with control rack (background), (B) LLSM without enclosure, (C) laser combiner without enclosure.