Kyle Roux, PhD

Scientist | Senior Director, Biomedical Sciences
Sanford Research
Pediatrics & Rare Diseases
(605) 312-6418

Dr. Roux received his PhD in Neuroscience at the University of Florida College of Medicine in 2004. Subsequently, Kyle performed postgraduate work at the University of Florida College of Medicine in the Department of Anatomy and Cell Biology, first as a Postdoctoral Associate and then as a Research Assistant Professor. Currently he is a faculty member at Sanford Research in the Enabling Technologies Group and principle investigator in the Roux Lab. To help reveal protein functions and mechanisms of disease Dr. Roux created the BioID method that generates a history of protein associations in living cells. BioID is a transformative method that has been adopted by hundreds of research groups worldwide to study a wide variety of proteins and biological processes including cancer, infectious disease, diabetes and rare diseases. The Roux laboratory uses BioID to investigate the structure and function of the mammalian nuclear envelope in health and disease.

Areas of expertise:
Proximity proteomics, BioID, nuclear envelope biology, mammalian cell biology

Selected publications:
Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ. An improved smaller biotin ligase for BioID proximity labeling. Molecular Biology of the Cell. (2016) Feb 24. pii: mbc.E15-12-0844

Birendra KC, May DG, Benson BV, Kim DI, Shivega WG, Ali MH, Faustino RS, Campos AR, Roux KJ. VRK2A is an A-type lamin-dependent nuclear envelope kinase that phosphorylates BAF. Molecular Biology of the Cell. (2017) Jun 21. pii: mbc.E17-03-0138. doi: 10.1091/mbc.E17-03-0138. [Epub ahead of print]

Kim DI, KC B, Zhu W, Motamedchaboki K, Doye V, Roux KJ. Probing nuclear pore complex architecture with proximity-dependent biotinylation. Proc Natl Acad Sci USA. (2014) Jun 17;111(24):E2453-61.

Kim DI and Roux KJ. Filling the void: Proximity-based labeling of proteins in living cells. Trends in Cell Biology. 2016 Nov;26(11):804-817. doi: 10.1016/j.tcb.2016.09.004. Epub (2016) Sep 22

Roux KJ, Kim DI, Burke B. BioID: A screen for protein-protein interactions. Current Protocols in Protein Science (2013) ePub Nov 5

Roux KJ, Kim DI, Raida M, Burke B (2012) A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. Journal of Cell Biology 196(6).

Horn HF, Kim DI, Wright G, Wong ESM, Stewart CL, Burke B, Roux KJ. Mammalian KASH5 couples meiotic chromosomes to the cytoskeleton. Journal of Cell Biology. (2013) Sep 30;202(7):1023-39.

Liu Q, Pante N, Misteli T, Elsagga M, Crisp M, Hodzic D, Burke B and Roux KJ. (2007) Functional association of Sun1 with nuclear pore complexes. Journal of Cell Biology 178:785-98

Roux KJ, Crisp M, Liu Q, Kim D, Kozlov S, Stewart CL, Burke B. (2009) Nesprin 4: A novel nuclear envelope protein induces kinesin-mediated cell polarization. Proceedings of the National Academy of Sciences 106(7): 2194-9

Stewart CL, Roux KJ and Burke B. (2007) Blurring the boundary: the nuclear envelope extends its reach. Science 318:1408-12

 

Return to Members main page.