Wenfeng An

Wenfeng An, PhD

Associate Professor
South Dakota State University
Pharmaceutical Sciences
(605) 688-4767

Dr. An focuses on the genetic and epigenetic regulation and functional impact of LINE-1 activities during germ cell development and cancer progression. His group employs innovative cell culture and animal models to investigate the role of jumping genes in developmental and disease processes.

Areas of expertise:
Cancer, epigenetics, mouse model, retrotransposon, spermatogenesis

Selected publications:
Newkirk, S.J., Lee, S., Grandi, F.C., Gaysinskaya, V., Rosser, J.M., Vanden Berg, N., Hogarth, C.A., Marchetto, M.C.N., Muotri, A.R., Griswold, M.D., Ye, P., Bortvin, A., Gage, F.H., Boeke, J.D. & An, W. Intact piRNA pathway prevents L1 mobilization in male meiosis. Proceedings of the National Academy of Sciences 114:E5635–E5644 (2017). DOI: 10.1073/pnas.1701069114. Commentary: Wang, P.J. Tracking LINE1 retrotransposition in the germline. Proceedings of the National Academy of Sciences 114:7194–7196 (2017)

Grandi, F.C., Rosser, J.M., Newkirk, S.J., Yin, J., Jiang, X., Xing, Z., Whitmore, L., Bashir, S., Ivics, Z., Izsvák, Z., Ye, P., Yu, Y.E. & An, W. Retrotransposition creates sloping shores: a graded influence of hypomethylated CpG islands on flanking CpG sites. Genome Research. 25:1135-1146 (2015). PMID: 25995269. PMCID: PMC4509998. DOI: 10.1101/gr.185132.114.

O'Donnell, K.A., An, W., Schrum, C.T., Wheelan, S.J., & Boeke, J.D. Controlled insertional mutagenesis using a LINE-1 (ORFeus) gene-trap mouse model. Proc Natl Acad Sci U S A. 110:E2706-13 (2013). PMID: 23818630. PMCID: PMC3718180. DOI: 10.1073/pnas.1302504110.

Xie, Y., Mates, L., Ivics, Z., Izsvák, Z., Martin, S.L. & An, W. Cell division promotes efficient retrotransposition in a stable L1 reporter cell line. Mobile DNA. 4:10 (2013). PMID: 23497436. PMCID: PMC3607998. DOI: 10.1186/1759-8753-4-10.

Grandi, F.C., Rosser, J.M. & An, W. LINE-1 derived poly(A) microsatellites undergo rapid shortening and create somatic and germline mosaicism in mice. Mol Biol Evol. 30:503-12 (2013). PMID: 23125228. PMCID: PMC3563966. DOI: 10.1093/molbev/mss251.

An, W., Dai, L., Niewiadomska, A.M., Yetil, A., O'Donnell, K.A., Han, J.S., & Boeke, J.D. Characterization of a synthetic human LINE-1 retrotransposon ORFeus-Hs. Mobile DNA. 2:2 (2011). PMID: 21320307. PMCID: PMC3045867. DOI: 10.1186/1759-8753-2-2.

Xie, Y., Rosser, J.M., Thompson, T.L., Boeke, J.D. & An, W. Characterization of L1 retrotransposition with high-throughput dual-luciferase assays. Nucleic Acids Res, 39, e16 (2011). PMID: 21320307. PMCID: PMC3045867. DOI: 10.1186/1759-8753-2-2.

Rosser, J.M. & An, W. Repeat-induced gene silencing of L1 transgenes is correlated with differential promoter methylation. Gene, 456, 15-23 (2010). PMID: 20167267. PMCID: PMC3001326. DOI: 10.1016/j.gene.2010.02.005.

An, W., Han, J.S.,Schrum, C.M., Maitra, A., Koentgen, F. & Boeke, J.D. Conditional activation of a single-copy L1 transgene in mice by Cre. Genesis 46, 373-83 (2008). PMID: 18615728. PMCID: PMC2614868. DOI: 10.1002/dvg.20407.

An, W., Han, J.S., Wheelan, S.J., Davis, E.S., Coombes, C.E., Ye, P., Triplett, C. & Boeke, J.D. Active retrotransposition by a synthetic L1 element in mice. Proc Natl Acad Sci U S A 103, 18662-7 (2006). PMID: 18615728. PMCID: PMC2614868. DOI: 10.1002/dvg.20407.

 

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